vzdělávání a poradenství

Certifikovana metodika

SEDLÁK, Jiří, SEMERÁK, Matěj a REJLOVÁ, Martina

Certifikovaná metodika ozdravování rostlin ovocných druhů od fytoplazem pomocí biotechnologických metod chemoterapie a in vitro kultur

VÝZKUMNÝ A ŠLECHTITELSKÝ ÚSTAV OVOCNÁŘSKÝ HOLOVOUSY s.r.o., 2023

ISBN 978-80-87030-93-6

DOI 10.60615/cezm-kk60

Certifikovaná metodika má za cíl poskytnout uživatelům in vitro postupy, chemoterapeutické techniky s využitím antibiotických látek a metody kryoterapie pro získání fytoplazem prostého výchozího množitelského materiálu jádrovin a peckovin. Je založena i na PCR determinaci fytoplazem. V rámci metodiky jsou popsána i vhodná kultivační média s regulátory růstu pro multiplikaci a indukci kořenů v podmínkách in vitro u studovaných ovocných druhů. Popsána je rovněž fytotoxicita studovaných antibiotických látek. Pro testování a ozdravování jsme vybrali tržní odrůdy a krajové odrůdy domácího původu. Předpokládáme, že získané zdravé základní rostliny budou po opakovaném testování moderními molekulárními diagnostickými metodami PCR v budoucnu zahrnuty do systému certifikace zdravotního stavu výsadbového materiálu.

Klicova slova: jádroviny; peckoviny; fytoplazma; in vitro; chemoterapie; kryoterapie

STAHNOUT

Basic information about VŠÚO

RESEARCH AND BREEDING FRUIT INSTITUTE HOLOVOUSY s.r.o. has been engaged in research of fruit growing and the breeding of fruit crops continuously for almost seven decades. The research activity of the institute includes practically all fruit crops that are grown on the territory of the Czech Republic as market cultures. As part of the solution of research projects supported by various providers (MZe/ NAZV, MŠMT, GAČR, MK, TAČR), it creates almost all types of outputs defined by the Research Organizations' Results Evaluation Methodologies and submitted to the Register of Results Information. These are both results of a publishing nature and applied results. Researchers and scientists publish research results in impacted, peer-reviewed, as well as in other professional and popular journals. The organization has been publishing Scientific Work on Fruit Growing for 60 years. The journal publishes original scientific works from the fruit growing industry. It is a peer-reviewed journal included in the List of peer-reviewed non-impact journals (periodicals) published in the Czech Republic. It is cited in CA B Abstracts/Horticultural Science Abstracts, Plant Breeding Abstracts, AGRIS. The successfully commercialized results include legally protected varieties, so far almost 85 varieties of individual fruit species have been entered and registered, other varieties are still undergoing the registration process. A number of varieties were granted a protection of rights in the Czech Republic and subsequently in the European Union. In particular, there is a great interest in cherry varieties in the world, two varieties have been granted a US Plant Patent. Furthermore, several results in the field of pilot plant and verified technologies were realized and contractually handed over to the user in VŠÚO Holovousy in the last five-year period. An important component of the transfer of research results into practice are growing methodologies, which are passed on to users - professionals - professional fruit growers

Company executives
Ing. Tomáš Zmeškal
Ing. Jaroslav Vácha

Companions
Ing. Jan Blažek, CS c.
Ing. Josef Kosina, CS c.
Ing. Václav Ludvík
Ing. František Paprštein, CS c.
Jaroslav Muška
Ing. Radoslav Potůček
SEMPRA PRAHA a.s.

Company Supervisory Board
Ing. Josef Kosina
Mgr. Vladimír Samek
Mgr. Hana Vránová